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Galactose Oxidase
GaO catalyzes the oxidation of primary alcohols to aldehydes and subsequently reduces dioxygen to hydrogen peroxide

Galactose Oxidase

Item Id: FSB0008
Your Price:
$159.00
Price and Availability
Amount
50 U [$159]
Quantity
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  • Description
  • Supporting Documents
  • Protocols
  • FAQ
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Galactose oxidase (GaO) is a recombinantly expressed copper activated enzyme derived from Dactylium dendroides that catalyzes the stereospecific oxidation of D-isomer primary alcohols to aldehydes and hydrogen peroxide (1-3). GaO has a range of substrates that include D-galactose and polysaccharides, glycolipids, or glycoproteins with D-galactose at their non-reducing end.

GaO has been shown to be useful in a number of biotechnology and medical applications which include monitoring of galactose in blood and urine (4), paper strengthening additives (5), test strips for cancer diagnosis (6), biosensors and lactose detection (7-8), dental care (9) and hair coloring and waving (10).



Properties

Quantity50 Units
Specific Activity>1750 U/mg
Purity>95% by SDS-PAGE
Molecular Weight68.9 kDa (Calculated)
Extinction coefficient1 mg/ml = 1.79/cm path length (Calculated) at 280 nm
Theoretical pI8.34
Storage Buffer50 mM acetate pH 7.2, 10 μM CuSO4
Unit Definition One unit will produce a ΔA425 of 1.0 per min at pH 6.0 at 25 C, in a peroxidase and o-Tolidine system.



Supplied Reagents

  • Enzyme (stored in 50 mM Acetate - pH 7.2, 10 μM CuSO4)


References

  1. Avigard, G. et al. (1962) J. Biol. Chem. 237, 2736‐2743.
  2. Schlegel, C.M. et al. (1968) Carbohydr. Res. 7, 193‐199.
  3. Kosman, D. J. (1994) in Copper Proteins and Copper Enzymes Vol. 2, 1‐26.
  4. Roth, H. et al. (1965) Anal. Biochem. 10, 32-52.
  5. Hartmans, S. et al. (2004) Hemicelluloses: Sci. Technol. 864, 360-371.
  6. Vucenik, I. et al. (2001) Anticancer Res. 21, 1247-1255.
  7. Brahim, S. et al. (2002) Biosens. Bioelectron 17, 973-981.
  8. Sharma, S. K. et al. (2006) Sens. Actuators B 119, 15-19.
  9. Lis, M. et al. (1997) Antimicrob. Agents Chemother. 41, 999-1003.
  10. Tsuijino, Y. et al. (1991) J. Soc. Cosmet. Chem. 42, 273-282.



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