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Real Time Blues™- Hydrogen Peroxide/Peroxidase Assay
Real Time Blues™ (RTB) - Peroxide/Peroxidase Assay

Real Time Blues™- Hydrogen Peroxide/Peroxidase Assay

Item Id: FSB3001
Your Price:
$175.00
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High Sensitivity and Stable Color


The versatility of real-time data collection with the convenience of an endpoint assay


Real-time BluesTM (RTB) - hydrogen peroxide/peroxidase-coupled assays are derived from a superior formulation of 3,3’,5,5’-tetramethylbenzidine (TMB). In the presence of peroxide and peroxidase the aromatic amine TMB contained is converted to produce a typically unstable blue chromophore intermediate with a peak absorbance at a wavelength of 650 nm. The RTB reagent was created to stabilize the blue chromophore for up to several days. Stable coloration enables assays to be performed in real-time or in endpoint experiments.


The RRB reagent is able to detect picomole quantities of hydrogen peroxide and picogram quantities of horseradish peroxidase (HRP) activity using a visible light spectrophotometer or by photographic analysis. The adaptable formula provides utility in a broad range of pH (3.5-9.0), temperature (4 – 60°C), and applications. RTB assays have been developed for applications in glycobiology, immunology, and cellular ROS (Reactive oxygen species) assays.


RTB - Hydrogen Peroxide/Peroxidase Detection Kit: This kit contains reagents for assaying at least 500 peroxide or peroxidase detection solutions in a 96-well microplate with a total sample volume of 100 μL per well.


Kit Specifications


Kit Contents

Product Specifications

Method

Colorimetric or
photographic

Detection limit

Peroxide ~0.8 μM or 80 pmoles
Peroxidase ~0.2 μU/mL or 0.8 pg HRP

pH Range

3.5 to 9.0

Assay mode

Real-time or endpoint

Absorbance maximum

650 nm real-time, 450 nm endpoint

Reagent

Kit Component

Quantity

1

5X reaction buffer

10 mL

2

RTB reagent

3.2 mL

3

*Horseradish peroxidase

12.5 U

4

Hydrogen peroxide standard

500 µL






Supporting DocumentsFormat
Product Manual
Material Safety Data Sheet


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