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α(2-3)-Neuraminidase
NanH hydrolyses terminal sialic acid linkages

α(2-3)-Neuraminidase

Item Id: FSB0034
Your Price:
$89.00
Price and Availability
Amount
1 Units [$89]
5 Units [$299]
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  • Description
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Neuraminidase H (NanH) is an Exo-α-sialidase derived from Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 and recombinantly expressed in E. coli. NanH cleaves terminal sialic acid from oligosaccharides, glycoproteins, glycolipids, and synthetic substrates and prefers α(2-3) linkages ~250-fold over α(2-6) linkages.


Properties

Quantity1 Unit
Specific Activity670 μmol [3' Sialyl-lactose]/min * 1/mg
Purity>95% by SDS-PAGE
Molecular Weight45,672 Daltons (Calculated)
Extinction coefficient1 mg/ml = 1.25/cm path length (Calculated) at 280 nm
Theoretical pI8.72
Storage Buffer100 mM acetate pH 5.5, 25 mM NaCl
Units One unit is defined as the amount of enzyme that releases 1 μmol of terminal α-sialic acid from 3' sialyl-lactose per minute.
Specific Activity 3'sialyl-lactose - 5 ng/mL Neuraminidase, 1.5 mM 3’sialyl-lactose, 80 µL, 0.2 mg/mL BSA, 20 mM sodium acetate pH 5.5, 25 mM NaCl, 37 C. Initial activity was measured in 20 min time intervals from 0 to 140 min using our Thiobarbituric Acid Assay Kit (FSB3002).
Conditions6' sialyl-lactose - 50 ng/mL Neuraminidase 1.5 mM 6’sialyl-lactose, 80 µL, 0.2 mg/mL BSA, 20 mM sodium acetate pH 5.5, 25 mM NaCl, 37 C. Initial activity was measured in 20 min time intervals from 0 to 140 min using our Thiobarbituric Acid Assay Kit. (FSB3002).



Supplied Reagents

  • Enzyme (stored in 100 mM Acetate - pH 5.5, 25 mM NaCl)
  • 10X Reaction Buffer (0.1 M sodium Acetate – pH 5.5, 250 mM NaCl)




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