Foresight Biosciences, Inc Home | My Account | Customer Service | Shopping Cart Shopping Cart - 0 item(s) / Total: $0
Home > Enzymes > Exoglycosidases

α(2-3,6)-Neuraminidase
Neuraminidase hydrolyses terminal sialic acid linkages

α(2-3,6)-Neuraminidase

Item Id: FSB0001
Your Price:
$74.00
Price and Availability
Amount
10 Units [$74]
30 Units [$149]
Quantity
Email a friend Email a friend
  • Description
  • Supporting Documents
  • Protocols
  • FAQ
  • Related Products


Recombinant Exo-α-sialidase from Clostridium perfringens


Affinity purified, robust enzyme for terminal sialic acid removal.

Neuraminidase is the small Exo-α-sialidases derived from Clostridium perfringens. Recombinantly expressed in E. coli, this neuraminidase hydrolyses terminal sialic acid linkages from oligosaccharides, glycoproteins, glycolipids, and homopolymers of N-acetylneuraminic acid and synthetic substrates. Product specificity is α(2-3) > α(2-6) >> α(2-8).
 

Properties

Quantity≥ 10 Units
Specific Activity520 μmol [3' Sialyl lactose]/min * 1/mg
Purity>95% by SDS-PAGE
Molecular Weight42,566.4 Daltons (Calculated)
Extinction coefficient1 mg/ml = 1.94/cm path length (Calculated) at 280 nm
Theoretical pI5.99
StorageFormat: Liquid
Buffer: 100 mM acetate pH 5.5, 25 mM NaCl
Temperature: 4°C.
Stability: 1 year
Units One unit is defined as the amount of enzyme that releases 1 μmol of terminal α-sialic acid from 3' sialyl-lactose per minute.
Specific Activity 3' sialyl-lactose - 5 ng/mL Neuraminidase, 1.5 mM 3’sialyl-lactose, 80 µL, 0.2 mg/mL BSA, 20 mM sodium acetate pH 5.5, 25 mM NaCl, 37 C. Initial activity was measured in 20 min time intervals from 0 to 140 min using our Thiobarbituric Acid Assay Kit (FSB3002).
Conditions6' sialyl-lactose - 50 ng/mL Neuraminidase 1.5 mM 6’sialyl-lactose, 80 µL, 0.2 mg/mL BSA, 20 mM sodium acetate pH 5.5, 25 mM NaCl, 37 C. Initial activity was measured in 20 min time intervals from 0 to 140 min using our Thiobarbituric Acid Assay Kit. (FSB3002).



Supplied Reagents

  • Enzyme (stored in 100 mM Acetate - pH 5.5, 25 mM NaCl)
  • 10X Reaction Buffer (0.1 M sodium Acetate – pH 5.5, 250 mM NaCl)




Related Products
3'-Sialyllactose sodium salt
3'-Sialyllactose sodium salt
Your Price: $50.00
6'-Sialyllactose sodium salt
6'-Sialyllactose sodium salt
Your Price: $50.00
Neuraminidase Assay
Neuraminidase Assay
Your Price: $300.00
PNGase F
PNGase F
Your Price: $119.00
  
Recently Viewed Items