The Real Time Blues™ (RTB) - D-glucose detection assay
Optimized for glycobiology applications
The Real Time Blues™ (RTB) - D-glucose detection assay can be used to quantify the D-glucose concentration in an unknown sample. In this assay, glucose is oxidized by glucose oxidase to D-gluconate and hydrogen peroxide. Subsequently, horseradish peroxidase is used to convert our RTB reagent and the resulting hydrogen peroxide into a stable blue product that can be measured optically. The amount of glucose is then calculated by comparing the D-glucose unknown sample to the D-glucose standard.
What's Included
This kit provides enough reagents for conducting 100 assays of D-glucose in a 96-well optical plate using sample volumes of 100 µL per well. In this format as little as 2 nmoles of D-glucose can be quantified.
Principle | Specifications | Technical Info |
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 | > Quantitative colorimetric | > Robust reagent |
> Real-time or Endpoint | > 1 mg/L D-glucose detection |
> Broad range linearity | |
> Stable coloration | |
> pH: 3.0 to 9.0 | |
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References
- Holland V.R., Saunders B.C., Rose F.L., Walpole A.L..”A safer substitute for benzidine in the detection of blood”. Tetrahedron 30: 3299 (1974).
- Josephy, P.D., Eling, T., and Mason, R.P. “The Horseradish Peroxidase-catalyzed Oxidation of 3,5,3’,5’-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.” J. Biol. Chem. 257, 3669-3675 (1982).
- Marquez, L.A. and Dunford, H.B. Mechanism of the oxidation of 3,5,3’,5’-tetramethylbenzidine by myeloperxidase determined by transient-and steady-state kinetics. Biochemistry 36, 9349-9355 (1997).