The Real Time Blues™ (RTB) - D-glucose detection assay
Optimized for glycobiology applications
The Real Time Blues™ (RTB) - D-glucose detection assay can be used to quantify the D-glucose concentration in an unknown sample. In this assay, glucose is oxidized by glucose oxidase to D-gluconate and hydrogen peroxide. Subsequently, horseradish peroxidase is used to convert our RTB reagent and the resulting hydrogen peroxide into a stable blue product that can be measured optically. The amount of glucose is then calculated by comparing the D-glucose unknown sample to the D-glucose standard.
What's Included
This kit provides enough reagents for conducting 100 assays of D-glucose in a 96-well optical plate using sample volumes of 100 µL per well. In this format as little as 2 nmoles of D-glucose can be quantified.
| Principle | Specifications | Technical Info |
|---|
 | > Quantitative colorimetric | > Robust reagent |
| > Real-time or Endpoint | > 1 mg/L D-glucose detection |
| > Broad range linearity | |
| > Stable coloration | |
| > pH: 3.0 to 9.0 | |
| |
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