Foresight Biosciences, Inc Home | My Account | Customer Service | Shopping Cart Shopping Cart - 0 item(s) / Total: $0

RTB D-Glucose Detection Assay
RTB D-Glucose Detection Assay

RTB D-Glucose Detection Assay

Item Id: FSB3100
Your Price:
$125.00
Quantity
Email a friend Email a friend
  • Description
  • Technical Information
  • Protocols
  • FAQ
  • Related Products

The Real Time Blues™ (RTB) - D-glucose detection assay


Optimized for glycobiology applications


The Real Time Blues™ (RTB) - D-glucose detection assay can be used to quantify the D-glucose concentration in an unknown sample. In this assay, glucose is oxidized by glucose oxidase to D-gluconate and hydrogen peroxide. Subsequently, horseradish peroxidase is used to convert our RTB reagent and the resulting hydrogen peroxide into a stable blue product that can be measured optically. The amount of glucose is then calculated by comparing the D-glucose unknown sample to the D-glucose standard.

What's Included

This kit provides enough reagents for conducting 100 assays of D-glucose in a 96-well optical plate using sample volumes of 100 µL per well. In this format as little as 2 nmoles of D-glucose can be quantified.


Principle Specifications Technical Info
> Quantitative colorimetric> Robust reagent
> Real-time or Endpoint> 1 mg/L D-glucose detection
> Broad range linearity
> Stable coloration
> pH: 3.0 to 9.0



References

 

  1. Holland V.R., Saunders B.C., Rose F.L., Walpole A.L..”A safer substitute for benzidine in the detection of blood”. Tetrahedron 30: 3299 (1974).
  2. Josephy, P.D., Eling, T., and Mason, R.P. “The Horseradish Peroxidase-catalyzed Oxidation of 3,5,3’,5’-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.” J. Biol. Chem. 257, 3669-3675 (1982).
  3. Marquez, L.A. and Dunford, H.B. Mechanism of the oxidation of 3,5,3’,5’-tetramethylbenzidine by myeloperxidase determined by transient-and steady-state kinetics. Biochemistry 36, 9349-9355 (1997).

 




Supporting DocumentsFormat
Product Manual
Material Safety Data Sheet


Related Products
RTB Lactose/D-Glucose Detection Assay
RTB Lactose/D-Glucose Detection Assay
Your Price: $175.00
RTB Lactose/D-Galactose Detection Assay
RTB Lactose/D-Galactose Detection Assay
Your Price: $225.00
RTB Sucrose/D-Glucose Detection Assay
RTB Sucrose/D-Glucose Detection Assay
Your Price: $175.00
Glucose Oxidase
Glucose Oxidase
Your Price: $79.00
  
Recently Viewed Items