The Real Time Blues™ (RTB) - Lactose/D-galactose detection assay
Optimized for glycobiology applications
The Real Time Blues™ (RTB) - Lactose/D-galactose detection assay can be used to quantify the lactose or D-galactose concentration in an unknown sample. In this assay, lactose is hydrolysed by β-galactosidase to produce total D-glucose and D-galactose. Subsequently, galactose oxidase converts the D-galactose to D-galacto-hexodialdose and hydrogen peroxide. Finally, horseradish peroxidase is used to convert our RTB reagent and the resulting hydrogen peroxide into a stable blue product that can be measured optically. The amount of lactose is then calculated by subtracting the free D-galactose detection from the total D-galactose detection (sample with and without β-galactosidase hydrolysis).
What's Included
This kit provides enough reagents for conducting 100 assays of lactose or D-galactose in a 96-well optical plate using sample volumes of 100 µL per well. In this format as little as 2 nmoles of D-galactose or 4 nmoles of lactose can be quantified.
| Principle | Specifications | Technical Info |
|---|
 | > Quantitative colorimetric | > Robust reagent |
| > Real-time or Endpoint | > 2 mg/L lactose detection |
| > Broad range linearity | > 1 mg/L D-galactose detection |
| > Stable coloration | |
| > pH: 3.0 to 9.0 | |
| |
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