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RTB Lactose/D-Glucose Detection Assay
RTB Lactose/D-Glucose Detection Assay

RTB Lactose/D-Glucose Detection Assay

Item Id: FSB3101
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The Real Time Blues™ (RTB) - Lactose/D-glucose detection assay

Optimized for glycobiology applications

The Real Time Blues™ (RTB) - Lactose/D-glucose detection assay can be used to quantify the lactose or D-glucose concentration in an unknown sample. In this assay, lactose is hydrolysed by β-galactosidase to produce total D-glucose and D-galactose. Subsequently, glucose oxidase converts the D-glucose to D-gluconate and hydrogen peroxide. Finally, horseradish peroxidase is used to convert our RTB reagent and the resulting hydrogen peroxide into a stable blue product that can be measured optically. The amount of lactose is then calculated by subtracting the free D-glucose detection from the total D-glucose detection (sample with and without β-galactosidase hydrolysis).

What's Included

This kit provides enough reagents for conducting 100 assays of lactose or D-glucose in a 96-well optical plate using sample volumes of 100 µL per well. In this format as little as 2 nmoles of D-glucose or 4 nmoles of lactose can be quantified.

Principle Specifications Technical Info
> Quantitative colorimetric> Robust reagent
> Real-time or Endpoint> 2 mg/L lactose detection
> Broad range linearity> 1 mg/L D-glucose detection
> Stable coloration
> pH: 3.0 to 9.0



  1. Holland V.R., Saunders B.C., Rose F.L., Walpole A.L..”A safer substitute for benzidine in the detection of blood”. Tetrahedron 30: 3299 (1974).
  2. Josephy, P.D., Eling, T., and Mason, R.P. “The Horseradish Peroxidase-catalyzed Oxidation of 3,5,3’,5’-tetramethylbenzidine. Free radical and charge-transfer complex intermediates.” J. Biol. Chem. 257, 3669-3675 (1982).
  3. Marquez, L.A. and Dunford, H.B. Mechanism of the oxidation of 3,5,3’,5’-tetramethylbenzidine by myeloperxidase determined by transient-and steady-state kinetics. Biochemistry 36, 9349-9355 (1997).
  4. American Association of Cereal Chemists. 1983. Approved Methods of the AACC, 8th edition. Methods 80-68, 80-31 and 80-50. The Association; St. Paul, MN.


Supporting DocumentsFormat
Product Manual
Material Safety Data Sheet

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